Assessment of Mutations on the Fitness & Virulence of Acinetobacter baumanni
The Gram-negative bacterium Acinetobacter baumanni (AB) is an important opportunistic pathogen because of its propensity for prolonged survival in a desiccated state, prevalence in hospital-acquired infections, & ability to acquire multi-drug resistance (MDR). The MDR phenotype has expanded alarmingly with resistance reports to nearly all clinically relevant antibiotics. AB re-gained notoriety during OIF /OEF, often isolated from infections following severe traumatic wounds sustained in military combat.
The virulence attributes of AB are not well understood, and combined MDR trends represents a critical need for this military-relevant pathogen. The aim of this project is to better understand the virulence of AB, focusing on its surface structures. Clones from a transposon library with disruptions in putative outer membrane proteins will be identified and tested to determine whether the mutations attenuate virulence and other characteristics compared against its parental wild-type strain.
Methodologies employed include classical bacteriology and molecular biology.
CONUS AIAD opportunities aligned with this project include research with the Department of Wound Infections from Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD
Point of Contact:
COL Carl Brinkley